Marvel is all about the female spider-heroes these days.
Well, I've got one more for them:
The Spider Queen!!
Yet the question stands--just who the heck is the Spider Queen?
She only appeared in 3 stories, and this is the only one I've read. She did do a lot of punching. She did a lot of swinging, a lot of lurking, and...
The Spider Queen is public domain now...Marvel should grab her (after my tiny finder's fee, of course), and retcon her into their universe as the first of the Spider-heroes, and she was Peter's great aunt, and he found her formula for the webbing, and...well, I'll let Dan Slott or someone else do the heavy lifting.
From The Eagle #2 (1941)